林敏，中國農(nóng)業(yè)科學(xué)院生物技術(shù)研究所所長，研究員、博士生導(dǎo)師。
2016年6月12日，農(nóng)業(yè)部向社會(huì)公示了第五屆農(nóng)業(yè)轉(zhuǎn)基因生物安全委員會(huì)委員名單，林敏為國家農(nóng)業(yè)轉(zhuǎn)基因生物安全委員成員。
人物經(jīng)歷
主要履歷
1985年畢業(yè)于四川大學(xué)生物系生化專業(yè)；
1991年獲中國農(nóng)科院研究生院生物物理專業(yè)農(nóng)學(xué)博士學(xué)位；
1988.8--1993.8，中國農(nóng)科院原子能所生物技術(shù)室；
1993.8--1994.5，法國巴斯德研究所生物技術(shù)系博士后，從事聯(lián)合固氮基因克隆和表達(dá)研究；
1997.6--1998.4，荷蘭瓦赫寧根荷蘭農(nóng)業(yè)科學(xué)院植保所訪問學(xué)者，從事基因工程固氮微生物環(huán)境釋放生物安全評價(jià)技術(shù)研究；
2002.12-至今，中國農(nóng)業(yè)科學(xué)院生物技術(shù)研究所研究員、博士生導(dǎo)師、所長。
社會(huì)兼職
曾任“十五”國家“863”計(jì)劃生物領(lǐng)域生物工程技術(shù)主題專家組專家、國家自然科學(xué)基金委員會(huì)第十一屆生命科學(xué)部評審組專家。2009年國家杰出青年基金獲得者。現(xiàn)為國家生物基因工程安全委員會(huì)委員、中國農(nóng)業(yè)生物技術(shù)學(xué)會(huì)常務(wù)副理事長、中國生物工程學(xué)會(huì)副理事長。
研究方向
主要從事水稻聯(lián)合固氮微生物的功能基因組和合成生物學(xué)研究，以及抗除草劑、耐輻射、抗鹽耐旱等特殊功能微生物基因資源的開發(fā)利用。
主要貢獻(xiàn)
近年來主持和參加國家863計(jì)劃“特殊微生物基因資源開發(fā)利用”、國家杰出青年科學(xué)基金項(xiàng)目“固氮施氏假單胞菌A1501功能基因組”、國家973計(jì)劃“聯(lián)合固氮菌固氮基因網(wǎng)絡(luò)調(diào)控與酶催化分子機(jī)理”、國家轉(zhuǎn)基因重大專項(xiàng)“抗逆和抗除草劑關(guān)鍵基因克隆及功能驗(yàn)證”、科技部國際合作重大課題“轉(zhuǎn)基因成分檢測技術(shù)的研究”等課題。
近年來開展了系統(tǒng)深入的研究工作并取得如下進(jìn)展：
（1）完成了斯氏假單胞菌感染A1501全基因組測序，是目前國際上完成全基因組序列分析的第一例聯(lián)合固氮菌。利用基因芯片、適時(shí)定量PCR和非極性突變株構(gòu)建等方法，鑒定了一系列可能參與細(xì)菌氮信號(hào)傳導(dǎo)或保持最佳固氮水平的新基因，開展了固氮系統(tǒng)進(jìn)化、固氮網(wǎng)絡(luò)調(diào)控、以及固氮調(diào)控元件和功能模塊的合成生物學(xué)系統(tǒng)研究。
（2）從極端環(huán)境如新疆戈壁灘、核爆炸中心區(qū)沙漠土壤取樣，建立了微生物生態(tài)基因組文庫并開始進(jìn)行了元基因組測序，鑒定了多株耐輻射微生物屬新種，開展了耐輻射新種D. gobiensis I-0全基因組、轉(zhuǎn)錄組和蛋白組分析以及極端抗性機(jī)制的研究工作。獲得一系列具有潛在抗逆能力如耐鹽/抗旱的功能基因。耐輻射總體調(diào)控基因irrE在大腸桿菌中的表達(dá)增強(qiáng)細(xì)胞的耐輻射能力和提高細(xì)胞抗氧化能力，將irrE基因?qū)霟煵荨⒂筒酥校黠@提高了植物耐鹽抗旱性能。
（3）克隆了一系列新型草甘膦抗性EPSPS編碼基因。構(gòu)建了含草膦抗性EPSP合酶基因和草甘膦N-乙轉(zhuǎn)移酶（GAT）基因的單價(jià)和雙價(jià)植物表達(dá)質(zhì)粒，通過膿桿菌介導(dǎo)方法導(dǎo)入不具草甘膦抗性的煙草、油菜、玉米品種中，通過花粉管通道法轉(zhuǎn)入棉花中，分別獲得了能夠耐受草甘膦的轉(zhuǎn)基因棉花、油菜、水稻、玉米、大豆、小麥等。轉(zhuǎn)基因棉花和油菜已進(jìn)入生物安全評價(jià)的中間試驗(yàn)階段，轉(zhuǎn)基因抗草甘膦玉米已批準(zhǔn)進(jìn)入環(huán)境釋放。
申請與抗草甘膦基因和耐鹽抗旱基因相關(guān)的國內(nèi)專利二十余項(xiàng)，國際PCT專利4項(xiàng)，其中獲國內(nèi)專利4項(xiàng), 美國專利1項(xiàng)。培養(yǎng)碩士研究生12名，指導(dǎo)博士研究生15名。
主要論著
在PNAS， PLoS ONE，AEM， IJSEM, Microbiology，現(xiàn)代生物出版集團(tuán) Genomics等國際刊物上發(fā)表SCI論文40余篇，主編專著3部。
1)Li S, Yan Y, Zhou Z, Yu H, Zhan Y, Zhang W, Chen M, Lu W, Ping S, Lin M. 2010. Single amino acid residue changes in subsite -1 of levansucrase from 發(fā)酵單胞菌屬 mobilis 10232 strongly influence the enzyme activities and products. 摩爾 Biol Rep. DOI 10.1007/s11033-010-，0379，5
2)Li, D., Y. Yan, S. Ping, M. Chen, W. Zhang, L. Li, W. Lin, L. Gen.G, W. Liu, W. Lu, and M. Lin. 2010. Genome-wide investigation and functional characterization of the beta-ketoadipate pathway in the nitrogen-fixing and root-associated bacterium 假單胞菌屬 stutzeri A1501. BMC Microbiol 10:36
3)Yan, Y., S. Ping, J. Peng, Y. Han, L. Li, J. Yang, Y. Dou, Y. Li, H. Fan, Y. Fan, D. Li, Y. Zhan, M. Chen, W. Lu, W. Zhang, Q. Cheng, Q. Jin, and M. Lin. 2010. Global transcriptional analysis of nitrogen fixation and ammonium repression in root-associated 假單胞菌屬 stutzeri A1501. 現(xiàn)代生物出版集團(tuán) Genomics 11:11
4)Zhang, W., H. H. Zhu, M. Yuan, Q. Yao, R. Tang, M. Lin, S. Z. Yang, Z. K. Li, and M. 金鐘大 2010. 微桿菌屬 radiodurans sp. nov., a UV radiation-resistant bacterium isolated from soil. Int J Syst Evol Microbiol 60:2665-70
5)Zhao, Z., X. Ma, L. Li, W. Zhang, S. Ping, M. Q. Xu, and M. Lin. 2010. Protein cyclization enhanced thermostability and exopeptidase-resistance of green fluorescent protein. J Microbiol Biotechnol 20:460-6
6)Ma, R., Y. Zhang, H. Hong, W. Lu, M. Lin, M. Chen, and W. Zhang. 2010. Improved Osmotic Tolerance and 乙醇 Production of Ethanologenic Escherichia coli by IrrE, a Global Regulator of Radiation-Resistance of Deinococcus radiodurans. Curr Microbiol
7)peng, Z., Y. Yan, Y. Xu, M. Takeo, H. Yu, Z. Zhao, Y. Zhan, W. Zhang, M. Lin, and M. Chen. 2010. Improvement of an E. coli bioreporter for monitoring trace amounts of phenol by deletion of the inducible sigma54-dependent promoter. Biotechnol Lett 32:1265-70
8)Jiang, R. P., D. J. Tang, X. L. Chen, Y. Q. He, J. X. Feng, B. L. Jiang, G. T. Lu, M. Lin, and J. L. Tang. 2010. Identification of four novel small non-coding RNAs from 黃單胞菌屬 campestris pathovar campestris. bmc Genomics 11:316
9)pan, J., J. Wang, Z. Zhou, Y. Yan, W. Zhang, W. Lu, S. Ping, Q. Dai, M. Yuan, B. Feng, X. Hou, Y. Zhang, R. Ma, T. Liu, L. Feng, L. Wang, M. Chen, and M. Lin. 2009. IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus. PLoS One 4:e4422
10)Yuan, M., W. Zhang, S. Dai, J. Wu, Y. Wang, T. Tao, M. Chen, and M. Lin. 2009. 異常球菌屬 gobiensis sp. nov., an extremely radiation-resistant bacterium. Int J Syst Evol Microbiol 59:1513-7
11)Gen.G, L., M. Chen, Q. Liang, W. Liu, W. Zhang, S. Ping, W. Lu, Y. Yan, W. Wang, M. Takeo, and M. Lin. 2009. Functional analysis of a putative regulatory gene, tadR, involved in 苯胺 degradation in Delftia tsuruhatensis AD9. Arch Microbiol 191:603-14
12)Zhan, Y., H. Yu, Y. Yan, S. Ping, W. Lu, W. Zhang, M. Chen, and M. Lin. 2009. Benzoate catabolite repression of the phenol degradation in Acinetobacter calcoaceticus PHEA-2. Curr Microbiol 59:368-73
13)Li, L., W. Lu, Y. Han, S. Ping, W. Zhang, M. Chen, Z. Zhao, Y. Yan, Y. Jiang, and M. Lin. 2009. A novel RPMXR motif among class II 5-enolpyruvylshikimate-3-磷酸鹽 synthases is required for enzymatic activity and 草甘膦 resistance. J Biotechnol 144:330-6
14)Yan, Y., J. Yang, Y. Dou, M. Chen, S. Ping, J. Peng, W. Lu, W. Zhang, Z. Yao, H. Li, W. Liu, S. He, L. Gen.G, X. Zhang, F. Yang, H. Yu, Y. Zhan, D. Li, Z. Lin, Y. Wang, C. Elmerich, M. Lin, and Q. Jin 2008. Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated 假單胞菌屬 stutzeri A1501. Proc Natl Acad Sci U S A 105:7564-9
15)Zhao, Z., W. Lu, B. Dun, D. Jin, S. Ping, W. Zhang, M. Chen, M. Q. Xu, and M. Lin. 2008. Purification of green fluorescent protein using a two-intein system. Appl Microbiol Biotechnol 77:1175-80
16)Dou Y., Yan Y, Ping S, Lu W, Chen M, Zhang W, Wang Y, Jin Q and Lin M. Expression profile analysis if the oxygen response in the nitrogen-fixing Pseudomonas stutzeri A1501 by genome-wide microarray. Chinese science bulletin, 2008,53(8):1197-1204
17)Zhan, Y., H. Yu, Y. Yan, M. Chen, W. Lu, S. Li, Z. peng, W. Zhang, S. Ping, J. Wang, and M. Lin. 2008. Genes involved in the benzoate catabolic pathway in 不動(dòng)桿菌屬 calcoaceticus PHEA-2. Curr Microbiol 57:609-14
18)Liu, X., J. Wu, W. Zhang, S. Ping, W. Lu, M. Chen, and M. Lin. 2008. Resistance of Deinococcus radiodurans to mutagenesis is facilitated by pentose 磷酸鹽 pathway in the mutS1 mutant background. Curr Microbiol 57:66-71
19)Wang, Z., M. Chen, Y. Xu, S. Li, W. Lu, S. Ping, W. Zhang, and M. Lin. 2008. An 乙醇tolerant recombinant 埃希氏菌屬 coli expressing 發(fā)酵單胞菌屬 mobilis pdc and adhB genes for enhanced ethanol production from xylose. Biotechnol Lett 30:657-63
20)Li, S. Y., M. Chen, G. Li, Y. L. Yan, H. Y. Yu, Y. H. Zhan, Z. X. peng, J. Wang, and M. Lin. 2008. Amino acid substitutions of His296 alter the catalytic properties of 發(fā)酵單胞菌屬 mobilis 10232 levansucrase. Acta Biochim Pol 55:201-6
21)Liang, A., J. Sha, W. Lu, M. 金鐘大, L. Li, D. Jin, Y. Yan, J. Wang, S. Ping, W. Zhang, Y. Wang, and M. Lin. 2008. A single residue mutation of 5-enoylpyruvylshikimate-3-磷酸鹽 synthase in 假單胞菌屬 stutzeri enhances resistance to the herbicide 草甘膦 Biotechnol Lett 30:1397-401
22)He, S., M. Chen, Z. Xie, Y. Yan, H. Li, Y. Fan, S. Ping, M. Lin, and C. Elmerich. 2008. Involvement of GlnK, a PII protein, in control of nitrogen fixation and ammonia assimilation in 假單胞菌屬 stutzeri A1501. Arch Microbiol 190:1-10
23)Dun, B. Q., X. J. Wang, W. Lu, Z. L. Zhao, S. N. Hou, B. M. Zhang, G. Y. Li, T. C. Evans, Jr., M. Q. Xu, and M. Lin. 2007. Reconstitution of 草甘膦 resistance from a split 5-enolpyruvyl shikimate-3-磷酸鹽 synthase gene in 埃希氏菌屬 coli and transgenic tobacco. Appl Environ Microbiol 73:7997-8000
24)Jin, D., W. Lu, S. Ping, W. Zhang, J. Chen, B. Dun, R. Ma, Z. Zhao, J. Sha, L. Li, Z. Yang, M. Chen, and M. Lin. 2007. Identification of a new gene encoding EPSPs with high 草甘膦 resistance from the metagenomic library. Curr Microbiol 55:350-5
25)Liu, Z., Z. pan, Y. Xu, Z. 越南盾, Z. Yang, and M. Lin. 2006. Cloning and expression of a 5-enolpyruvylshikimate-3-磷酸鹽 synthase gene from Halomonas variabilis. 脫氧核糖核酸 Seq 17:208-14
26)Xie, Z., Y. Dou, S. Ping, M. Chen, G. Wang, C. Elmerich, and M. Lin. 2006. Interaction between NifL and NifA in the nitrogen-fixing 假單胞菌屬 stutzeri A1501. Microbiology 152:3535-42
27)Liang, Q., M. Takeo, M. Chen, W. Zhang, Y. Xu, and M. Lin. 2005. Chromosome-encoded gene cluster for the metabolic pathway that converts 苯胺 to TCA-cycle intermediates in Delftia tsuruhatensis AD9. Microbiology 151:3435-46
28)Yan, Y., J. Yang, L. Chen, F. Yang, J. 越南盾, Y. Xue, X. Xu, Y. Zhu, Z. Yao, M. Lin, Y. Wang, and Q. Jin. 2005. Structural and functional analysis of denitrification genes in 假單胞菌屬 stutzeri A1501. Sci China C Life Sci 48:585-92
29)Sun, Y. C., Y. C. Chen, Z. X. Tian, F. M. Li, X. Y. Wang, J. Zhang, Z. L. Xiao, M. Lin, N. Gilmartin, D. N. Dowling, and Y. P. Wang. 2005. Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China. Appl Environ Microbiol 71:4771-6
30)Desnoues, N., M. Lin, X. Guo, L. Ma, R. Carreno-Lopez, and C. Elmerich. 2003. Nitrogen fixation genetics and regulation in a 假單胞菌屬 stutzeri strain associated with rice. Microbiology 149:2251-62
31)Xu, Y., M. Chen, W. Zhang, and M. Lin. 2003. Genetic organization of genes encoding phenol hydroxylase, benzoate 1,2-dioxygenase alpha subunit and its regulatory proteins in 不動(dòng)桿菌屬 calcoaceticus PHEA-2. Curr Microbiol 46:235-40
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