Fukai Bao，男，博士生導師，云南騰沖人，祖籍浙江杭州，生于1963年，醫學微生物學家和熱帶蟲媒傳染病專家。
基本介紹
Fukai Bao
Profile Title:
Frank(Fukai) Bao,MD/PhD, The Professor, department chairman
Education:
1980~1985 MD, Kunming Medical College,Kunming, Yunnan, China
1985~1987 MS, Kunming Medical College,Kunming, Yunnan, China
2002~2005 Postdoc, 耶魯大學 University, New Haven, CT, USA
Research highlight
節肢動物門borne infectious diseases remain an international public health threat. To prevent or treat these illnesses, we are developing new strategies based on an understanding of the intimate relationship between the vector, pathogen, and mammalian host. My laboratory focuses on three kinds of arthropod-transmitted diseases to explore these interactions. Lyme disease, caused by the spirochete Borrelia burgdorferi, is the most common 真蜱目borne illness in the United States. Anaplasma, mainly Anaplasma phagocytophilum, an obligate intracellular bacterium that persists within neutrophils, causes human granulocytic anaplasmosis and is primarily transmitted by Ixodes ticks. Finally, richettsiaes, which are expanding their territory throughout the world. In my lab, Lyme disease was used as a model to show that immunization with B. burgdorferi outer surface protein (Osp) A is protective against infection. This led to an 美國食品藥品監督管理局approved human Lyme disease vaccine. Surprisingly, antibodies elicited by the vaccine enter the tick when the arthropod feeds, and then destroy OspA-producing B. burgdorferi in the vector. As OspA is normally down-regulated by spirochetes during transmission from ticks to humans, this demonstrates an unusual mechanism of vaccine action—the targeting of an antigen expressed by a microbe within the vector to protect humans from infection.
My laboratory is interested in how pathogens may use 節肢動物門 products to facilitate infection of the mammalian host. As B. burgdorferi migrate from ticks to humans, the spirochetes influence the expression of tick salivary gland genes. B. burgdorferi then bind to some of the induced salivary proteins, such as Salp15, and use the tick proteins to evade mammalian immune responses. These discoveries led to the paradigm that microbes may use specific 節肢動物門 molecules to enable successful infection of the host—a triangular interaction at the ephemeral pathogen-vector-host interface that occurs while an arthropod is feeding on a 脊椎動物 I am also determining whether, to be successfully acquired by the vector, pathogens within mammals use 節肢動物門 proteins that are secreted into the host. For example, ticks engorging on vertebrates induce recruitment of inflammatory cells to the bite site. For efficient migration to the vector, pathogens must traffic through this complex environment while avoiding the deleterious effects of immune cells. We are studying whether these paradigms—initially established with B. burgdorferi- are applicable to other 節肢動物門borne infectious agents, including A. phagocy- tophilum and West Nile 病毒
My lab is also developing techniques to identify the entire panel of vector genes that are induced or repressed by pathogens, and assays to delineate the complete profile of 節肢動物門 proteins that microbes adhere to, or otherwise interact with. We are also assessing whether colonization of arthropods by specific infectious agents is a mutualistic, rather than parasitic, relationship. Understanding these interactions may lead to new ways to interrupt the life cycle of 節肢動物門borne pathogens and new vaccine strategies against these diseases. Molecular evaluation of vector-borne pathogen infections: Our research is focused on characterizing vector-borne pathogens, particularly tick-transmitted rickettsial disease agents, Ehrlichia chaffeensis and Ehrlichia canis. We use the tools of molecular biology, immunology, 動物界 models and cell culture systems. E. chaffeensis is the agent causing human monocytic ehrlichiosis (HME). It also infects dogs, white-tailed deer, goats and coyotes. E. canis is primarily responsible for the canine monocytic ehrlichiosis. It is endemic throughout the world, except Australia. These closely related pathogens remain in both 節肢動物門 and 脊椎動物 hosts for long periods of 時間 This strategy may aid them in their parasitic lifestyle. Determining the molecular basis for persistence by these bacteria of the genus Ehrlichia will be important in order to accomplish our long range goals of developing effective methods of control against these tick-borne pathogens. Our research is focused on understanding the pathogen evasion mechanisms. Specifically, we study to define the protective host-immune response needed to eliminate the infection. We also study to define how these rickettsiales evade host responses in both 脊椎動物 and tick hosts in order to support their life cycle.
Recent main publications
１） Pal U, Wang P, Bao F(寶福凱，并列第一作者), Yang X, Samanta S, Robert Schoen , Wormser GP, Schwartz I, and Erol Fikrig. Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis. J Exp Med. 2008;205: 133-141.(影響因子： 2007年15.6)
２） Utpal Pal, Xin Li, Tian Wang, Ruth R. Montgomery, Nandhini Ramamoorthi, Aravinda M. deSilva, Fukai Bao（寶福凱）, Xiaofeng Yang, Marc Pypaert, Deepti Pradhan, Fred S. Kantor, Sam Telford, John F. Anderson, and Erol Fikrig. TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi. Cell. 2004；119:457-468.（影響因子：32.４）
３） Nandhini Ramamoorthi, Sukanya Narasimhan , Utpal Pal, Fukai Bao（寶福凱）, Xiaofeng F. Yang ,Juan Anguita, Michael V. Norgard, Fred S. Kantor, John F. Anderson, Raymond A. Koski and Erol Fikrig. The Lyme disease agent exploits a tick protein to infect the mammalian host. Nature. 2005;436:573-577.（影響因子：32.7）
４）Yongli Zhang, Fukai Bao, Jianjian Hu, et al. Antimicrobial lignans and triterpenoids from Rostellularia prcumbens. 植物界 Medica. 2007; 73:1596-1599. (SCI收錄)
５）Hengxing Liang, Fukai Bao, Xiaoping Dong , Rui Tan , Caijun Zhang , Qing Lu and Yongxian Cheng. Antibacterial thymol Derivatives Isolated from Centipeda minima. Molecules. 2007; 12:小行星16061613.（SCI收錄）
６）Heng-Xing Liang, Fu-Kai Bao, Xiao-Ping Dong, Hua-Jie Zhu, Xiao-Jie Lua, Ming Shi, Qing Lu, and Yong-Xian Cheng. Two New Antibacterial Sesquiterpenoids from 石胡荽屬 minima. 化學 & Biodiversity. 2007; 4:2810-2816. （SCI收錄）
７）Ren-Qiang Mei , Qing Lu, Yan-Fen Hu, Hai-Yang Liu, Fu-Kai Bao, Yu Zhang, Yong-Xian Cheng. Three New Polyyne (=聚乙炔) Glucosides from the Edible Roots of 黨參屬 cordifolioidea. Helvetica Chimica Acta. 2008; 91:90 – 96. （SCI收錄） ８
）Fengwei Bai, Tian Wang, Utpal Pal, Fukai Bao, L. Hannah Gould and Erol Fikrig. Prevention of lethal murine West Nile 病毒 infection using 核糖核酸 interference. Journal of Infectious Diseases. 191:1148-1154, 2005. (SCI收錄)
９) 寶福凱, Erol Fikrig。伯氏疏螺旋體與嗜吞噬細胞無漿體混合感染的實驗研究。熱帶醫學雜志2007；７：195-200.
10) 寶福凱, Erol Fikrig。伯氏疏螺旋體與嗜吞噬細胞無漿體感染抑制Th1 型細胞因子產生。中國熱帶醫學。2007；７：1061-1063.
11) 寶福凱。蜱傳病原體的混合感染研究進展。中國熱帶醫學。2007，7（1）：112～114
12）寶福凱。伯氏疏螺旋體與萊姆病研究進展。熱帶醫學雜志。2007；7:1125-1127
13）寶福凱。立克次體目微生物的系統分類進展。中國人獸共患病學報。2007;23:1262-1264
14）寶福凱, 柳愛華, 張愉。天克菌膠囊體內抗結核作用實驗研究。熱帶醫學雜志。2006，6（3）：975-977
15）柳愛華，寶福凱（通訊作者）,張 杰, 舒潔。地錦草提取物抗口腔病原菌作用的體外研究。中國熱帶醫學。2007 ，7 （12 ）：2194,2217
16) 柳愛華，李畢賢，施明，寶福凱（通訊作者）。類物質YK—1和YK—2體外抗菌作用的研究。中國病原生物學雜志。2008, 3（3）：176-177
17）梁恒興, 寶福凱, 董曉萍, 呂 青, 程永現。鵝不食草中具有抗菌活性的三萜類成分。云南植物研究。 2007 , 29 (4) : 479～482
18) 寶福凱， Erol Fikrig. The pathogen-vector interactions during Borrelia burgdorferi transmission（英文）.中國媒介生物學與控制雜志。2008, 19（3）：264－268
19)寶福凱, Erol Fikrig。The Joint-specific Expression Profile of Borrelia burgdorfri in the Murine Hosts（英文）. 科技通報。2008,24（6）:832-838
20)寶福凱，柳愛華，馬海濱。人類無形體病的病原學和流行病學研究進展。熱帶醫學雜志，2008，8（11）：1193-1195
21) 寶福凱，柳愛華，馬海濱，吳娜，施 明. 萊姆關節炎發病機理研究進展. 中國病原生物學雜志。2009,在版
22) 柳愛華，程 玲，張才軍，陳 芳，施 明，陳 亮，沈培清，劉汝文，角建林，李瑪琳，寶福凱（通訊作者）。野生中緬樹伯氏疏螺旋體感染的Touchdown PCR檢測。中國熱帶醫學。2009 ，9（3）：在版
23) 寶福凱，柳愛華。小鼠模型中伯氏疏螺旋體組織載量的定量研究。中國媒介生物學與控制雜志。2009, 20（3）：在版
24）柳愛華，寶福凱（通訊作者）。新發現的抑制性細胞因子——白細胞介素35。中國免疫學雜志。2009，第3期，在版
25）李 莉，寶福凱（通訊作者）。白介素-32及其與炎癥性疾病的關系研究進展。現代 免疫學雜志。2009，修回 26）寶福凱，柳愛華，施 明，馬海濱。人類無漿體病的臨床表現、診斷和治療研究進展。熱帶醫學雜志。200；9:在版
27）陳 亮，寶福凱(通訊作者)。結核分枝桿菌異煙耐藥基因與耐藥機制研究進展。中國人獸共患病學報。2009;25:在版
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